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Tuesday 26 March 2019

Science DNA Extraction



Aim
To extract DNA from a kiwi fruit.


Method

First we had to cut a kiwi fruit in half then scoop the half into a snap lock bag and then we mushed a kiwifruit so there were no lumps then we had to pour the  kiwifruit onto a cloth and funnel then drain the liquid into the funnel and let sit for 5 mins then add methanol down the side of the test tube and let sit for mins and then you will see the kiwi fruit DNA float to the top after some time.


Using a spatula scoop 1/2 kiwifruit into bag.Add 25 ml water+ a pinch of salt

Mush until a thick soup

Pour over cloth into funnel into beaker

pour liquid into test tube.Add dish washing liquid+ swirl.Allow to sit for 5 mins.

Tilt test tube on 45 degree angle and slowly pour 10 ml of ethanol down the side

Allow to sit.

Results




In the test tube there is a cloudy yellowish color


Water helped break kiwifruit apart salt helped clump the DNA
Ethanol Helped see it as it sits on top. This helped us find the kiwi fruit DNA
The salt helped the DNA group together and the ethanol helped float it to the top so we could see it


So in the end the experiment was a success because the kiwi fruit DNA floated to the top




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